› 5fkk

X-ray diffraction
1.75Å resolution

TetR(D) N82A mutant in complex with anhydrotetracycline and magnesium

Released:
DOI: 10.2210/pdb5fkk/pdb
PDB entry 5fkk coloured by chain, front view.
PDB entry 5fkk coloured by chain, side view.
PDB entry 5fkk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Modular organisation of inducer recognition and allostery in the tetracycline repressor.
Werten S, Schneider J, Palm GJ, Hinrichs W
FEBS J 283 2102-14 (2016)
PMID: 27028290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0ACT4 (Residues: 3-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand PG4 1 x PG4
Ligand SO4 1 x SO4
Ligand CL 9 x CL
Ligand TDC 1 x TDC
Ligand MG 1 x MG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P43212
Unit cell:
a: 68.231Å b: 68.231Å c: 179.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.254
Expression system: Escherichia coli K-12

Quick links

Citations

11 citation in other articles

Evolution-guided engineering of small-molecule biosensors.
Snoek et al. (2020)
10 more

1 mention without citation

Modular organisation of inducer recognition and allostery in the tetracycline repressor.
Werten et al. (2016)