› 5g26

X-ray diffraction
2.42Å resolution

Unveiling the Mechanism Behind the in-meso Crystallization of Membrane Proteins

Released:
DOI: 10.2210/pdb5g26/pdb
PDB entry 5g26 coloured by chain, front view.
PDB entry 5g26 coloured by chain, side view.
PDB entry 5g26 coloured by chain, top view.
Source organism: Escherichia coli O157
Primary publication:
The nanoscience behind the art of in-meso crystallization of membrane proteins.
Zabara A, Meikle TG, Newman J, Peat TS, Conn CE, Drummond CJ
Nanoscale 9 754-763 (2017)
PMID: 27976759

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intimin Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.79 KDa
Source organism: Escherichia coli O157
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43261 (Residues: 208-449; Coverage: 26%)
Gene names: ECs4559, L0025, Z5110, eae, eaeA
Sequence domains: Inverse autotransporter, beta-domain
Structure domains: Inverse autotransporter, beta-domain

Ligands and Environments

2 bound ligands:
Ligand 97N 3 x 97N
Ligand 97M 1 x 97M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 115.944Å b: 119.925Å c: 39.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

On the thermotropic and magnetotropic phase behavior of lipid liquid crystals containing magnetic nanoparticles.
Mendozza et al. (2018)
4 more

1 mention without citation

Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains.
Zabara et al. (2018)