› 5g2d

X-ray diffraction
1.8Å resolution

The crystal structure of light-driven chloride pump ClR (T102N) mutant at pH 4.5.

Released:
DOI: 10.2210/pdb5g2d/pdb
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PDB entry 5g2d coloured by chain, front view.
PDB entry 5g2d coloured by chain, side view.
PDB entry 5g2d coloured by chain, top view.
Source organism: Nonlabens marinus S1-08
Primary publication:
Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif.
Kim K, Kwon SK, Jun SH, Cha JS, Kim H, Lee W, Kim JF, Cho HS
OpenAccess logo Nat Commun 7 12677 (2016)
PMID: 27554809

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.5 KDa
Source organism: Nonlabens marinus S1-08
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W8VZW3 (Residues: 1-272; Coverage: 100%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

4 bound ligands:
Ligand OLA 8 x OLA
Ligand RET 1 x RET
Ligand PEG 1 x PEG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C
Spacegroup: C2
Unit cell:
a: 103.252Å b: 49.447Å c: 78.402Å
α: 90° β: 131.89° γ: 90°
R-values:
R R work R free
0.177 0.175 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.
Inoue K. (2021)
5 more

1 mention without citation

Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif.
Kim et al. (2016)

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