› 5gm9

X-ray diffraction
1.36Å resolution

Crystal structure of a glycoside hydrolase in complex with cellobiose

Released:
DOI: 10.2210/pdb5gm9/pdb
PDB entry 5gm9 coloured by chain, front view.
PDB entry 5gm9 coloured by chain, side view.
PDB entry 5gm9 coloured by chain, top view.
Source organism: Thermothielavioides terrestris NRRL 8126
Primary publication:
Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase from Thielavia terrestris.
Gao J, Huang JW, Li Q, Liu W, Ko TP, Zheng Y, Xiao X, Kuo CJ, Chen CC, Guo RT
Enzyme Microb Technol 99 32-37 (2017)
PMID: 28193329

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cellulase Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 22.32 KDa
Source organism: Thermothielavioides terrestris NRRL 8126
Expression system: Komagataella pastoris
UniProt:
  • Canonical: G2QVH7 (Residues: 22-234; Coverage: 77%)
Gene name: THITE_2110957
Sequence domains: Glycosyl hydrolase family 45
Structure domains: RlpA-like domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P212121
Unit cell:
a: 46.337Å b: 54.315Å c: 84.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.164
Expression system: Komagataella pastoris

Quick links

Citations

8 citation in other articles

Phylogenetic re-evaluation of Thielavia with the introduction of a new family Podosporaceae.
Wang et al. (2019)
7 more