› 5go8

X-ray diffraction
2.21Å resolution

Linear tetra-ubiquitin

Released:
DOI: 10.2210/pdb5go8/pdb
PDB entry 5go8 coloured by chain, front view.
PDB entry 5go8 coloured by chain, side view.
PDB entry 5go8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Monomer/Oligomer Quasi-Racemic Protein Crystallography.
Gao S, Pan M, Zheng Y, Huang Y, Zheng Q, Sun D, Lu L, Tan X, Tan X, Lan H, Wang J, Wang T, Wang J, Liu L
J Am Chem Soc 138 14497-14502 (2016)
PMID: 27768314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-143316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
17 modified residues:
Ligand DNE 1 x DNE
Ligand DGN 6 x DGN
Ligand DIL 7 x DIL
Ligand DPN 2 x DPN
Ligand DVA 4 x DVA
Ligand DLY 7 x DLY
Ligand DTH 7 x DTH
Ligand DLE 9 x DLE
Ligand DGL 6 x DGL
Ligand DPR 3 x DPR
Ligand DSN 3 x DSN
Ligand DAS 5 x DAS
Ligand DSG 2 x DSG
Ligand DAL 2 x DAL
Ligand DAR 4 x DAR
Ligand DTY 1 x DTY
Ligand DHI 1 x DHI

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 29.395Å b: 56.178Å c: 38.721Å
α: 90° β: 91.44° γ: 90°
R-values:
R R work R free
0.228 0.222 0.322
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

6 review citations

Novel protein science enabled by total chemical synthesis.
Kent SBH. (2019)
5 more