› 5gw7

X-ray diffraction
2.2Å resolution

Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose

Released:
DOI: 10.2210/pdb5gw7/pdb
PDB entry 5gw7 coloured by chain, front view.
PDB entry 5gw7 coloured by chain, side view.
PDB entry 5gw7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
Miyazaki T, Nishikawa A, Tonozuka T
J Struct Biol 196 479-486 (2016)
PMID: 27688023

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucosidase YgjK Chains: A, B
Molecule details ›
Chains: A, B
Length: 760 amino acids
Theoretical weight: 85.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42592 (Residues: 24-783; Coverage: 100%)
Gene names: JW3051, b3080, ygjK
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand MG 2 x MG
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 56.74Å b: 137.069Å c: 81.599Å
α: 90° β: 100° γ: 90°
R-values:
R R work R free
0.157 0.154 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17 exo-α-glucosidases.
Miyazaki et al. (2020)
3 more