› 5gy2

X-ray diffraction
2.1Å resolution

Crystal structure of a complex between Bacillus subtilis flagellin and zebrafish Toll-like receptor 5

Released:
DOI: 10.2210/pdb5gy2/pdb
PDB entry 5gy2 coloured by chain, front view.
PDB entry 5gy2 coloured by chain, side view.
PDB entry 5gy2 coloured by chain, top view.
Source organisms:
Primary publication:
A conserved TLR5 binding and activation hot spot on flagellin.
Song WS, Jeon YJ, Namgung B, Hong M, Yoon SI
OpenAccess logo Sci Rep 7 40878 (2017)
PMID: 28106112

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109809 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
LRRNT domain-containing protein; TIR domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 51.17 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: B3DIN1 (Residues: 23-390; Coverage: 43%)
  • Canonical: Q4G1L2 (Residues: 126-200; Coverage: 30%)
Gene names: VLRB, tlr5b
Sequence domains:
Flagellin Chains: C, D
Molecule details ›
Chains: C, D
Length: 183 amino acids
Theoretical weight: 19.25 KDa
Source organism: Bacillus spizizenii str. W23
Expression system: Escherichia coli
UniProt:
  • Canonical: E0U497 (Residues: 54-230; Coverage: 64%)
Gene names: BSUW23_17335, hag
Sequence domains:
Structure domains: f41 fragment of flagellin, N-terminal domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 6 x NAG
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 78.146Å b: 54.281Å c: 165.98Å
α: 90° β: 98.62° γ: 90°
R-values:
R R work R free
0.197 0.195 0.236
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

18 review citations

Bacterial flagellin-a potent immunomodulatory agent.
Hajam et al. (2017)
17 more

5 mentions without citation

Structure of fish Toll-like receptors (TLR) and NOD-like receptors (NLR).
Sahoo BR. (2020)
4 more