› 5gy7

X-ray diffraction
1.43Å resolution

X-Ray structure of H243I mutant of UDP-Galactose 4-epimerase from E.coli:evidence for existence of open and closed active site during catalysis.

Released:
DOI: 10.2210/pdb5gy7/pdb
PDB entry 5gy7 coloured by chain, front view.
PDB entry 5gy7 coloured by chain, side view.
PDB entry 5gy7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Singh N, Tiwari P, Phulera S, Dixit A, Choudhury D

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 38.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P09147 (Residues: 1-338; Coverage: 100%)
Gene names: JW0742, b0759, galD, galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand UDP 2 x UDP
Ligand GOL 1 x GOL
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 47.11Å b: 91.16Å c: 80.26Å
α: 90° β: 99.87° γ: 90°
R-values:
R R work R free
0.135 0.134 0.16
Expression system: Escherichia coli

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