› 5h77

X-ray diffraction
3.2Å resolution

Crystal structure of the PKA-protein A fusion protein

Released:
DOI: 10.2210/pdb5h77/pdb
PDB entry 5h77 coloured by chain, front view.
PDB entry 5h77 coloured by chain, side view.
PDB entry 5h77 coloured by chain, top view.
Source organisms:
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
OpenAccess logo Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A; cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 90 amino acids
Theoretical weight: 10.31 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P13861 (Residues: 5-41, 44-45; Coverage: 10%)
  • Canonical: P38507 (Residues: 220-221, 224-268; Coverage: 10%)
Gene names: PKR2, PRKAR2, PRKAR2A, spa
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 71.588Å b: 135.378Å c: 88.988Å
α: 90° β: 108.77° γ: 90°
R-values:
R R work R free
0.242 0.239 0.284
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
11 other articles