› 5h7b

X-ray diffraction
3.1Å resolution

Crystal structure of a repeat protein with five Protein A repeat modules

Released:
DOI: 10.2210/pdb5h7b/pdb
PDB entry 5h7b coloured by chain, front view.
PDB entry 5h7b coloured by chain, side view.
PDB entry 5h7b coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
OpenAccess logo Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 26.97 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P38507 (Residues: 38-325; Coverage: 49%)
Gene name: spa
Sequence domains: B domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 80.89Å b: 45.64Å c: 82.14Å
α: 90° β: 105.72° γ: 90°
R-values:
R R work R free
0.271 0.269 0.315
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
9 other articles

2 mentions without citation

Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin.
Manne et al. (2020)
1 more