› 5hbw

X-ray diffraction
1.9Å resolution

RNA primer-template complex with 2-methylimidazole-activated monomer analogue

Released:
DOI: 10.2210/pdb5hbw/pdb
PDB entry 5hbw coloured by chain, front view.
PDB entry 5hbw coloured by chain, side view.
PDB entry 5hbw coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Unusual Base-Pairing Interactions in Monomer-Template Complexes.
Zhang W, Tam CP, Wang J, Szostak JW
OpenAccess logo ACS Cent Sci 2 916-926 (2016)
PMID: 28058281

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196931 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*(LCC)P*(TLN)P*(LCG)P*UP*AP*CP*A)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 7 nucleotides
Theoretical weight: 2.25 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PZG 2 x PZG
2 modified residues:
Ligand TLN 2 x TLN
Ligand LCG 2 x LCG

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 28.076Å b: 39.747Å c: 42.593Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.254
Expression system: Not provided

Quick links

Citations

1 review citation

The Narrow Road to the Deep Past: In Search of the Chemistry of the Origin of Life.
Szostak JW. (2017)
15 other articles

2 mentions without citation

Unusual Base-Pairing Interactions in Monomer-Template Complexes.
Zhang et al. (2016)
1 more