› 5hmn

X-ray diffraction
2.02Å resolution

Crystal structure of an aminoglycoside acetyltransferase HMB0005 from an uncultured soil metagenomic sample, unknown active site density modeled as polyethylene glycol

Released:
DOI: 10.2210/pdb5hmn/pdb
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PDB entry 5hmn coloured by chain, front view.
PDB entry 5hmn coloured by chain, side view.
PDB entry 5hmn coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Xu Z, Stogios PJ, Wawrzak Z, Skarina T, Yim V, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 159 amino acids
Theoretical weight: 17.77 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A059WV44 (Residues: 1-157; Coverage: 99%)
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand COA 5 x COA
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 58.27Å b: 62.756Å c: 253.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.221
Expression system: Escherichia coli BL21(DE3)

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