› 5hpm

X-ray diffraction
2.67Å resolution

Cetuximab Fab in complex with cyclic linked meditope

Released:
DOI: 10.2210/pdb5hpm/pdb
PDB entry 5hpm coloured by chain, front view.
PDB entry 5hpm coloured by chain, side view.
PDB entry 5hpm coloured by chain, top view.
Source organisms:
Primary publication:
Cyclization strategies of meditopes: affinity and diffraction studies of meditope-Fab complexes.
Bzymek KP, Ma Y, Avery KA, Horne DA, Williams JC
OpenAccess logo Acta Crystallogr F Struct Biol Commun 72 434-42 (2016)
PMID: 27303895

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-230006 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cetuximab Fab light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 213 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
Expression system: unidentified
Structure domains: Immunoglobulins
Cetuximab Fab heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 221 amino acids
Theoretical weight: 23.73 KDa
Source organism: Mus musculus
Expression system: unidentified
Structure domains: Immunoglobulins
Cyclic amidated, acetylated linked meditope Chains: E, F
Molecule details ›
Chains: E, F
Length: 11 amino acids
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand CY3 2 x CY3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 64.11Å b: 82.76Å c: 212.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.232
Expression systems:
  • unidentified
  • Not provided

Quick links

Citations

5 citation in other articles

Dual-Color Bioluminescent Sensor Proteins for Therapeutic Drug Monitoring of Antitumor Antibodies.
van Rosmalen et al. (2018)
4 more

1 mention without citation

Cyclization strategies of meditopes: affinity and diffraction studies of meditope-Fab complexes.
Bzymek et al. (2016)