› 5htc

X-ray diffraction
1.5Å resolution

Crystal structure of haspin (GSG2) in complex with bisubstrate inhibitor ARC-3372

Released:
DOI: 10.2210/pdb5htc/pdb
PDB entry 5htc coloured by chain, front view.
PDB entry 5htc coloured by chain, side view.
PDB entry 5htc coloured by chain, top view.
Source organisms:
Primary publication:
Co-crystal structures of the protein kinase haspin with bisubstrate inhibitors.
Lavogina D, Kestav K, Chaikuad A, Heroven C, Knapp S, Uri A
Acta Crystallogr F Struct Biol Commun 72 339-45 (2016)
PMID: 27139824

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214933 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TF76 (Residues: 465-798; Coverage: 42%)
Gene names: GSG2, HASPIN
Sequence domains: Haspin like kinase domain
Structure domains:
ARC-3372 INHIBITOR Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 804 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MPD 2 x MPD
Ligand NA 1 x NA
Ligand DMS 1 x DMS
Ligand 66M 1 x 66M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 77.97Å b: 78.903Å c: 81.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.171
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 citation in other articles

Knockdown of GSG2 inhibits prostate cancer progression in vitro and in vivo.
Yu et al. (2020)
1 more

1 mention without citation

Co-crystal structures of the protein kinase haspin with bisubstrate inhibitors.
Lavogina et al. (2016)