› 5hwa

X-ray diffraction
1.35Å resolution

Crystal Structure of MH-K1 chitosanase in substrate-bound form

Released:
DOI: 10.2210/pdb5hwa/pdb
PDB entry 5hwa coloured by chain, front view.
PDB entry 5hwa coloured by chain, side view.
PDB entry 5hwa coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Crystal structure of the GH-46 subclass III chitosanase from Bacillus circulans MH-K1 in complex with chitotetraose
Suzuki M, Saito A, Kobayashi M, Yokoyama T, Omiya S, Li J, Sugita K, Miki K, Saito JI, Ando A
Biomed. Biochim. Acta 1868 130549- (2024)

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitosanase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.99 KDa
Source organism: Niallia circulans
Expression system: Brevibacillus choshinensis
UniProt:
  • Canonical: P33673 (Residues: 43-301; Coverage: 100%)
Gene name: csn
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GCS
Carbohydrate polymer
3 bound ligands:
Ligand CAC 1 x CAC
Ligand ACY 3 x ACY
Ligand ZN 7 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 40.75Å b: 79.03Å c: 94.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.137 0.17
Expression system: Brevibacillus choshinensis

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