› 5hzi

X-ray diffraction
2.6Å resolution

Crystal structure of photoinhibitable Intersectin1 containing C450M mutant LOV2 domain

Released:
DOI: 10.2210/pdb5hzi/pdb
PDB entry 5hzi coloured by chain, front view.
PDB entry 5hzi coloured by chain, side view.
PDB entry 5hzi coloured by chain, top view.
Source organisms:
Primary publication:
Engineering extrinsic disorder to control protein activity in living cells.
Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM
Science 354 1441-1444 (2016)
PMID: 27980211

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intersectin-1; non-specific serine/threonine protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 58.31 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q15811 (Residues: 1230-1308, 1309-1580; Coverage: 20%)
  • Canonical: O49003 (Residues: 404-546; Coverage: 16%)
Gene names: ITSN, ITSN1, NPH1-1, SH3D1A
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 62.06Å b: 111.53Å c: 88.33Å
α: 90° β: 90.63° γ: 90°
R-values:
R R work R free
0.24 0.238 0.285
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Advances in protein structure prediction and design.
Kuhlman et al. (2019)
36 more

1 mention without citation

Engineering extrinsic disorder to control protein activity in living cells.
Dagliyan et al. (2016)