› 5i4b

X-ray diffraction
1.6Å resolution

Erwinia chrysanthemi L-asparaginase E63Q +S254N mutation + L-Aspartic acid

Released:
DOI: 10.2210/pdb5i4b/pdb
PDB entry 5i4b coloured by chain, front view.
PDB entry 5i4b coloured by chain, side view.
PDB entry 5i4b coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Design and Characterization of Erwinia Chrysanthemi l-Asparaginase Variants with Diminished l-Glutaminase Activity.
Nguyen HA, Su Y, Lavie A
J Biol Chem 291 17664-76 (2016)
PMID: 27354283

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139091 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 328 amino acids
Theoretical weight: 35.32 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P06608 (Residues: 23-348; Coverage: 100%)
Gene names: ansB, asn
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ASP 3 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I222
Unit cell:
a: 76.977Å b: 123.124Å c: 198.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.183
Expression system: Escherichia coli

Quick links

Citations

8 review citations

What makes a good new therapeutic L-asparaginase?
Beckett et al. (2019)
7 more

3 mentions without citation

Design and Characterization of Erwinia Chrysanthemi l-Asparaginase Variants with Diminished l-Glutaminase Activity.
Nguyen et al. (2016)
2 more