› 5iyx

X-ray diffraction
2.43Å resolution

Crystal structure of the Arabidopsis receptor kinase HAESA in complex with the peptide hormone IDA and the co-receptor SERK1

Released:
DOI: 10.2210/pdb5iyx/pdb
PDB entry 5iyx coloured by chain, front view.
PDB entry 5iyx coloured by chain, side view.
PDB entry 5iyx coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.
Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M
OpenAccess logo Elife 5 (2016)
PMID: 27058169

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155645 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Receptor-like protein kinase 5 Chain: A
Molecule details ›
Chain: A
Length: 658 amino acids
Theoretical weight: 71.68 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P47735 (Residues: 20-620; Coverage: 61%)
Gene names: At4g28490, F21O9.180, HAE, RLK5
Sequence domains:
Protein IDA Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.58 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8LAD7 (Residues: 56-69; Coverage: 28%)
Gene names: At1g68765, F14K14, IDA
Somatic embryogenesis receptor kinase 1 Chain: C
Molecule details ›
Chain: C
Length: 201 amino acids
Theoretical weight: 21.75 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q94AG2 (Residues: 24-213; Coverage: 31%)
Gene names: At1g71830, F14O23.21, F14O23_24, SERK1
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 4 x NAG
Ligand EDO 2 x EDO
1 modified residue:
Ligand HYP 1 x HYP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 74.507Å b: 100.456Å c: 142.759Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.234
Expression system: Trichoplusia ni

Quick links

Citations

24 review citations

Regulation of pattern recognition receptor signalling in plants.
Couto et al. (2016)
23 more

9 mentions without citation

Plant Leucine-Rich Repeat Receptor Kinase (LRR-RK): Structure, Ligand Perception, and Activation Mechanism.
Chakraborty et al. (2019)
8 more