› 5j6s

X-ray diffraction
2.8Å resolution

Crystal structure of Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) in complex with a hydroxamic derivative ligand

Released:
DOI: 10.2210/pdb5j6s/pdb
PDB entry 5j6s coloured by chain, front view.
PDB entry 5j6s coloured by chain, side view.
PDB entry 5j6s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structures of ERAP2 Complexed with Inhibitors Reveal Pharmacophore Requirements for Optimizing Inhibitor Potency.
Mpakali A, Giastas P, Deprez-Poulain R, Papakyriakou A, Koumantou D, Gealageas R, Tsoukalidou S, Vourloumis D, Mavridis IM, Stratikos E, Saridakis E
ACS Med Chem Lett 8 333-337 (2017)
PMID: 28337326

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Endoplasmic reticulum aminopeptidase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 967 amino acids
Theoretical weight: 111.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6P179 (Residues: 1-960; Coverage: 100%)
Gene names: ERAP2, LRAP
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand ZN 2 x ZN
Ligand NAG 11 x NAG
Ligand 6GA 2 x 6GA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 73.54Å b: 134.33Å c: 127.58Å
α: 90° β: 91.71° γ: 90°
R-values:
R R work R free
0.208 0.205 0.276
Expression system: Trichoplusia ni

Quick links

Citations

4 review citations

The Discovery of Insulin-Regulated Aminopeptidase (IRAP) Inhibitors: A Literature Review.
Georgiadis et al. (2020)
3 more

4 mentions without citation

Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families.
Pascual Alonso et al. (2023)
3 more