› 5jv5

X-ray diffraction
2.73Å resolution

Trypanosome brucei Hypoxanthine-guanine phosphoribosyltranferase in complex with Guanosine 5' monophosphate

Released:
DOI: 10.2210/pdb5jv5/pdb
PDB entry 5jv5 coloured by chain, front view.
PDB entry 5jv5 coloured by chain, side view.
PDB entry 5jv5 coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Crystal structures and inhibition of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltransferase.
Terán D, Hocková D, Česnek M, Zíková A, Naesens L, Keough DT, Guddat LW
OpenAccess logo Sci Rep 6 35894 (2016)
PMID: 27786284

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 24.22 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07010 (Residues: 1-210; Coverage: 100%)
Gene name: HGPRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand 5GP 2 x 5GP
Ligand SO4 4 x SO4
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21212
Unit cell:
a: 93.944Å b: 111.711Å c: 45.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.272
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metal-chelating agents against viruses and parasites.
Giannakopoulou et al. (2018)
1 more