› 5jzo

X-ray diffraction
2.5Å resolution

Structure of wild type amidase at high temperature at 2.5 Angstrom resolution

Released:
DOI: 10.2210/pdb5jzo/pdb
PDB entry 5jzo coloured by chain, front view.
PDB entry 5jzo coloured by chain, side view.
PDB entry 5jzo coloured by chain, top view.
Source organism: Vibrio cholerae O395
Entry authors: Chowdhury SR, Sen U

Function and Biology Details

Reaction catalysed: 
(R)-lactate = methylglyoxal + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101764 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-lactate dehydratase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 288 amino acids
Theoretical weight: 31.51 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3AFW5 (Residues: 1-286; Coverage: 100%)
Gene name: VC0395_0351
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 73.375Å b: 80.069Å c: 133.133Å
α: 90° β: 95.35° γ: 90°
R-values:
R R work R free
0.18 0.177 0.256
Expression system: Escherichia coli BL21(DE3)

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