› 5k0w

X-ray diffraction
2.61Å resolution

Crystal structure of the metallo-beta-lactamase GOB-18 from Elizabethkingia meningoseptica

Released:
DOI: 10.2210/pdb5k0w/pdb
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Source organism: Elizabethkingia meningoseptica
Primary publication:
Crystal Structure of the Metallo-β-Lactamase GOB in the Periplasmic Dizinc Form Reveals an Unusual Metal Site.
Morán-Barrio J, Lisa MN, Larrieux N, Drusin SI, Viale AM, Moreno DM, Buschiazzo A, Vila AJ
Antimicrob Agents Chemother 60 6013-22 (2016)
PMID: 27458232

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.37 KDa
Source organism: Elizabethkingia meningoseptica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4JRB6 (Residues: 1-290; Coverage: 100%)
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 8 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 64Å b: 48.51Å c: 88.94Å
α: 90° β: 100.1° γ: 90°
R-values:
R R work R free
0.195 0.192 0.242
Expression system: Escherichia coli

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Citations

2 review citations

Epidemiology of β-Lactamase-Producing Pathogens.
Bush et al. (2020)
1 more

9 mentions without citation

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
8 more

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