› 5k7j

X-ray diffraction
1.39Å resolution

Structure of designed zinc binding protein ZE2 bound to Zn2+

Released:
DOI: 10.2210/pdb5k7j/pdb
PDB entry 5k7j coloured by chain, front view.
PDB entry 5k7j coloured by chain, side view.
PDB entry 5k7j coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Probing the minimal determinants of zinc binding with computational protein design.
Guffy SL, Der BS, Kuhlman B
Protein Eng Des Sel 29 327-338 (2016)
PMID: 27358168

Function and Biology Details

Reaction catalysed: 
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-glycerol phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.46 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q06121 (Residues: 2-248; Coverage: 100%)
Gene names: SSO0895, trpC
Sequence domains: Indole-3-glycerol phosphate synthase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 41.718Å b: 79.029Å c: 74.077Å
α: 90° β: 95.7° γ: 90°
R-values:
R R work R free
0.198 0.196 0.23
Expression system: Escherichia coli BL21

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Citations

1 review citation

Step-by-step design of proteins for small molecule interaction: A review on recent milestones.
Pereira et al. (2021)
10 other articles