› 5kzd

X-ray diffraction
2.33Å resolution

N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus with bound sialic acid alditol

Released:
DOI: 10.2210/pdb5kzd/pdb
PDB entry 5kzd coloured by chain, front view.
PDB entry 5kzd coloured by chain, side view.
PDB entry 5kzd coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus USA300
Primary publication:
Structure and inhibition of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus.
North RA, Watson AJ, Pearce FG, Muscroft-Taylor AC, Friemann R, Fairbanks AJ, Dobson RC
FEBS Lett 590 4414-4428 (2016)
PMID: 27943302

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 293 amino acids
Theoretical weight: 33.08 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2G160 (Residues: 1-293; Coverage: 100%)
Gene names: SAOUHSC_00295, nanA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand RCJ 4 x RCJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 81.477Å b: 109.444Å c: 130.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.261
Expression system: Escherichia coli

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Citations

2 review citations

"Just a spoonful of sugar...": import of sialic acid across bacterial cell membranes.
North et al. (2018)
1 more