› 5l3v

X-ray diffraction
2.3Å resolution

Structure of the crenarchaeal SRP54 GTPase bound to GDP

Released:
DOI: 10.2210/pdb5l3v/pdb
PDB entry 5l3v coloured by chain, front view.
PDB entry 5l3v coloured by chain, side view.
PDB entry 5l3v coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structural Basis for Conserved Regulation and Adaptation of the Signal Recognition Particle Targeting Complex.
Wild K, Bange G, Motiejunas D, Kribelbauer J, Hendricks A, Segnitz B, Wade RC, Sinning I
J Mol Biol 428 2880-97 (2016)
PMID: 27241309

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle 54 kDa protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 33.17 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZE7 (Residues: 1-293; Coverage: 66%)
Gene names: SSO0971, srp54
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3221
Unit cell:
a: 92.168Å b: 92.168Å c: 148.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.222
Expression system: Escherichia coli

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Citations

5 review citations

Electrostatic Interactions in Protein Structure, Folding, Binding, and Condensation.
Zhou et al. (2018)
4 more