› 5l9j

X-ray diffraction
1.5Å resolution

Room temperature X-ray diffraction of tetragonal HEWL. First data set (0.31 MGy)

Released:
DOI: 10.2210/pdb5l9j/pdb
PDB entry 5l9j coloured by chain, front view.
PDB entry 5l9j coloured by chain, side view.
PDB entry 5l9j coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Uridine as a new scavenger for synchrotron-based structural biology techniques.
Crosas E, Castellvi A, Crespo I, Fulla D, Gil-Ortiz F, Fuertes G, Kamma-Lorger CS, Malfois M, Aranda MA, Juanhuix J
J Synchrotron Radiat 24 53-62 (2017)
PMID: 28009546

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P43212
Unit cell:
a: 79.244Å b: 79.244Å c: 37.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.113 0.111 0.162

Quick links

Citations

1 review citation

Synchrotron Radiation X-ray Diffraction Techniques Applied to Insect Flight Muscle.
Iwamoto H. (2018)
6 other articles

1 mention without citation

Robust total X-ray scattering workflow to study correlated motion of proteins in crystals.
Meisburger et al. (2023)