› 5lmc

X-ray diffraction
1.9Å resolution

Oxidized flavodiiron core of Escherichia coli flavorubredoxin, including the Fe-4SG atoms from its rubredoxin domain

Released:
DOI: 10.2210/pdb5lmc/pdb
PDB entry 5lmc coloured by chain, front view.
PDB entry 5lmc coloured by chain, side view.
PDB entry 5lmc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase.
Romão CV, Vicente JB, Borges PT, Victor BL, Lamosa P, Silva E, Pereira L, Bandeiras TM, Soares CM, Carrondo MA, Turner D, Teixeira M, Frazão C
J Mol Biol 428 4686-4707 (2016)
PMID: 27725182

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anaerobic nitric oxide reductase flavorubredoxin Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 54.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46877 (Residues: 1-479; Coverage: 100%)
Gene names: JW2680, b2710, flrD, norV, ygaI, ygaJ, ygaK
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand FEO 1 x FEO
Ligand FMN 1 x FMN
Ligand PO4 2 x PO4
Ligand CAC 1 x CAC
Ligand ACY 1 x ACY
Ligand GOL 1 x GOL
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P622
Unit cell:
a: 150.471Å b: 150.471Å c: 95.361Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.166 0.166
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
2 more

3 mentions without citation

On the Natural History of Flavin-Based Electron Bifurcation.
Baymann et al. (2018)
2 more