› 5mms

X-ray diffraction
2.8Å resolution

Human cystathionine beta-synthase (CBS) p.P49L delta409-551 variant

Released:
DOI: 10.2210/pdb5mms/pdb
PDB entry 5mms coloured by chain, front view.
PDB entry 5mms coloured by chain, side view.
PDB entry 5mms coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Clinically Relevant Variant of the Human Hydrogen Sulfide-Synthesizing Enzyme Cystathionine β-Synthase: Increased CO Reactivity as a Novel Molecular Mechanism of Pathogenicity?
Vicente JB, Colaço HG, Malagrinò F, Santo PE, Gutierres A, Bandeiras TM, Leandro P, Brito JA, Giuffrè A
OpenAccess logo Oxid Med Cell Longev 2017 8940321 (2017)
PMID: 28421128

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 408 amino acids
Theoretical weight: 44.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35520 (Residues: 1-408; Coverage: 74%)
Gene name: CBS
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 6 x PLP

Cofactor: Ligand HEM 6 x HEM
1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P1
Unit cell:
a: 86.174Å b: 86.783Å c: 97.786Å
α: 102.67° β: 103.07° γ: 111.19°
R-values:
R R work R free
0.184 0.182 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Hydrogen sulfide signaling in mitochondria and disease.
Murphy et al. (2019)
3 more

4 mentions without citation

Treatment of autosomal dominant hearing loss by in vivo delivery of genome editing agents.
Gao et al. (2018)
3 more