› 5suz

X-ray diffraction
1.84Å resolution

Domain-swapped dimer of human Dishevelled2 DEP domain: C-centered monoclinic crystal form crystallised from monomeric fraction

Released:
DOI: 10.2210/pdb5suz/pdb
PDB entry 5suz coloured by chain, front view.
PDB entry 5suz coloured by chain, side view.
PDB entry 5suz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Wnt Signalosome Assembly by DEP Domain Swapping of Dishevelled.
Gammons MV, Renko M, Johnson CM, Rutherford TJ, Bienz M
OpenAccess logo Mol Cell 64 92-104 (2016)
PMID: 27692984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Segment polarity protein dishevelled homolog DVL-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14641 (Residues: 416-509; Coverage: 13%)
Gene name: DVL2
Sequence domains: Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 117.785Å b: 59.601Å c: 30.322Å
α: 90° β: 93.64° γ: 90°
R-values:
R R work R free
0.22 0.217 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
29 more

1 mention without citation

Regulation of Dishevelled DEP domain swapping by conserved phosphorylation sites.
Beitia et al. (2021)