› 5t25

X-ray diffraction
1.99Å resolution

Kinetic, Spectral and Structural Characterization of the Slow Binding Inhibitor Acetopyruvate with Dihydrodipicolinate Synthase from Escherichia coli.

Released:
DOI: 10.2210/pdb5t25/pdb
PDB entry 5t25 coloured by chain, front view.
PDB entry 5t25 coloured by chain, side view.
PDB entry 5t25 coloured by chain, top view.
Source organism: Escherichia coli IAI1
Entry authors: Chooback L, Thomas LM, Karsten WE, Fleming CD, Seabourn P

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 31.55 KDa
Source organism: Escherichia coli IAI1
Expression system: Escherichia coli
UniProt:
  • Canonical: B7M7I1 (Residues: 1-292; Coverage: 100%)
Gene names: ECIAI1_2529, dapA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand LYS 2 x LYS
Ligand NA 10 x NA
1 modified residue:
Ligand 74P 2 x 74P

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 136.647Å b: 56.385Å c: 101.53Å
α: 90° β: 127.62° γ: 90°
R-values:
R R work R free
0.161 0.158 0.211
Expression system: Escherichia coli

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