› 5t73

X-ray diffraction
2.6Å resolution

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 336 amino acids
Theoretical weight: 36.37 KDa
Source organism: Staphylococcus aureus subsp. aureus MRSA252
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6GIL8 (Residues: 1-336; Coverage: 100%)
Gene names: SAR0828, gap, gapA, gapA1
Sequence domains:
Structure domains: Dihydrodipicolinate Reductase; domain 2

Ligands and Environments

1 bound ligand:
Ligand NA 8 x NA
1 modified residue:
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 95.692Å b: 111.456Å c: 128.063Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.26
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Role of Bacillithiol in Gram-Positive Firmicutes.
Chandrangsu et al. (2018)
5 more

2 mentions without citation

Redox regulation by reversible protein S-thiolation in Gram-positive bacteria.
Imber et al. (2019)
1 more