› 5tmp

X-ray diffraction
1.98Å resolution

COMPLEX OF E. COLI THYMIDYLATE KINASE WITH THE BISUBSTRATE INHIBITOR AZTP5A

Released:
DOI: 10.2210/pdb5tmp/pdb
PDB entry 5tmp coloured by chain, front view.
PDB entry 5tmp coloured by chain, side view.
PDB entry 5tmp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase.
Lavie A, Ostermann N, Brundiers R, Goody RS, Reinstein J, Konrad M, Schlichting I
Proc Natl Acad Sci U S A 95 14045-50 (1998)
PMID: 9826650

Function and Biology Details

Reaction catalysed: 
ATP + dTMP = ADP + dTDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-141510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate kinase Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A720 (Residues: 1-213; Coverage: 100%)
Gene names: JW1084, b1098, tmk, ycfG
Sequence domains: Thymidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand Z5A 1 x Z5A
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R32
Unit cell:
a: 152.1Å b: 152.1Å c: 75.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.253
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinases.
Van Rompay et al. (2000)
44 other articles

5 mentions without citation

Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase.
Lavie et al. (1998)
4 more