› 5trs

X-ray diffraction
3.08Å resolution

Structure of Mycobacterium tuberculosis proteasome in complex with N,C-capped dipeptide PKS2144

Released:
DOI: 10.2210/pdb5trs/pdb
PDB entry 5trs coloured by chain, front view.
PDB entry 5trs coloured by chain, side view.
PDB entry 5trs coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural Basis for the Species-Selective Binding of N,C-Capped Dipeptides to the Mycobacterium tuberculosis Proteasome.
Hsu HC, Singh PK, Fan H, Wang R, Sukenick G, Nathan C, Lin G, Li H
Biochemistry 56 324-333 (2017)
PMID: 27976853

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161585 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasome subunit alpha Chains: A, B, C, D, E, F, G, O, P, Q, R, S, T, U
Molecule details ›
Chains: A, B, C, D, E, F, G, O, P, Q, R, S, T, U
Length: 240 amino acids
Theoretical weight: 25.97 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 10-248; Coverage: 96%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: H, I, J, K, L, M, N, V, W, X, Y, Z, a, b
Molecule details ›
Chains: H, I, J, K, L, M, N, V, W, X, Y, Z, a, b
Length: 240 amino acids
Theoretical weight: 25.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:
Ligand 7HZ 14 x 7HZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 120.509Å b: 198.301Å c: 165.941Å
α: 90° β: 103.133° γ: 90°
R-values:
R R work R free
0.183 0.181 0.211
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Targeting the Proteostasis Network for Mycobacterial Drug Discovery.
Lupoli et al. (2018)
3 more

1 mention without citation

Structural Basis for the Species-Selective Binding of N,C-Capped Dipeptides to the Mycobacterium tuberculosis Proteasome.
Hsu et al. (2017)