› 5tux

X-ray diffraction
1.5Å resolution

crystal structure and light induced structural changes in orange carotenoid protein bound with echinenone

Released:
DOI: 10.2210/pdb5tux/pdb
PDB entry 5tux coloured by chain, front view.
PDB entry 5tux coloured by chain, side view.
PDB entry 5tux coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Photoactivation mechanism of a carotenoid-based photoreceptor.
Bandara S, Ren Z, Lu L, Zeng X, Shin H, Zhao KH, Yang X
Proc Natl Acad Sci U S A 114 6286-6291 (2017)
PMID: 28559328

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orange carotenoid-binding protein Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.51 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P74102 (Residues: 1-317; Coverage: 100%)
Gene name: slr1963
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ECH 1 x ECH
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 82.5Å b: 82.5Å c: 87.812Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.181
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Photoprotective, excited-state quenching mechanisms in diverse photosynthetic organisms.
Magdaong et al. (2018)
1 more

3 mentions without citation

Photoactivation mechanism of a carotenoid-based photoreceptor.
Bandara et al. (2017)
2 more