› 5upo

X-ray diffraction
1.7Å resolution

Crystal structure of BhGH81 mutant in complex with laminaro-pentaose

Released:
DOI: 10.2210/pdb5upo/pdb
PDB entry 5upo coloured by chain, front view.
PDB entry 5upo coloured by chain, side view.
PDB entry 5upo coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
The quaternary structure of beta-1,3-glucan contributes to its recognition and hydrolysis by a multimodular family 81 glycoside hydrolase
Pluvinage B, Fillo A, Massel P, Boraston AB
Structure (2017)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucan endo-1,3-beta-D-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 753 amino acids
Theoretical weight: 85.28 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KG76 (Residues: 28-779; Coverage: 76%)
Gene name: BH0236
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand PO4 11 x PO4
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 65.32Å b: 96.48Å c: 157.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.158
Expression system: Escherichia coli BL21(DE3)

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