› 5uqn

X-ray diffraction
2.06Å resolution

Clostridium difficile Toxin B (TcdB) glucosyltransferase domain in complex with U2F

Released:
DOI: 10.2210/pdb5uqn/pdb
PDB entry 5uqn coloured by chain, front view.
PDB entry 5uqn coloured by chain, side view.
PDB entry 5uqn coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
Clostridium difficile toxin glucosyltransferase domains in complex with a non-hydrolyzable UDP-glucose analogue.
Alvin JW, Lacy DB
J Struct Biol 198 203-209 (2017)
PMID: 28433497

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcdB Chain: A
Molecule details ›
Chain: A
Length: 563 amino acids
Theoretical weight: 65.36 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: P18177 (Residues: 1-543; Coverage: 23%)
Gene names: tcdB, toxB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand U2F 1 x U2F
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P41212
Unit cell:
a: 62.39Å b: 62.39Å c: 328.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The role of toxins in Clostridium difficile infection.
Chandrasekaran et al. (2017)
3 other articles

3 mentions without citation

Structural basis for selective modification of Rho and Ras GTPases by Clostridioides difficile toxin B.
Liu et al. (2021)
2 more