› 5uzz

Solution NMR

Structure of wild type pre-miR21 apical loop

Released:
DOI: 10.2210/pdb5uzz/pdb
PDB entry 5uzz coloured by chain, ensemble of 10 models, front view.
PDB entry 5uzz coloured by chain, ensemble of 10 models, side view.
PDB entry 5uzz coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
A Macrocyclic Peptide Ligand Binds the Oncogenic MicroRNA-21 Precursor and Suppresses Dicer Processing.
Shortridge MD, Walker MJ, Pavelitz T, Chen Y, Yang W, Varani G
ACS Chem Biol 12 1611-1620 (2017)
PMID: 28437065

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-166188 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
14-mer Peptide Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.81 KDa
Source organism: synthetic construct
Expression system: Not provided
pre-miR21 Chain: A
Molecule details ›
Chain: A
Length: 31 nucleotides
Theoretical weight: 9.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand DPR 1 x DPR

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 30%
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

12 review citations

Post-transcriptional control of miRNA biogenesis.
Michlewski et al. (2019)
11 more

1 mention without citation

Design of RNA-targeting macrocyclic peptides.
Walker et al. (2019)