› 5vjb

X-ray diffraction
2.1Å resolution

Guanidine-II riboswitch P2 hairpin dimer with 5-bromoU substitution from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5vjb/pdb
PDB entry 5vjb coloured by chain, front view.
PDB entry 5vjb coloured by chain, side view.
PDB entry 5vjb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis for ligand binding to the guanidine-II riboswitch.
Reiss CW, Strobel SA
OpenAccess logo RNA 23 1338-1343 (2017)
PMID: 28600356

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197009 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*GP*CP*GP*GP*GP*GP*AP*CP*GP*AP*CP*CP*CP*(5BU)P*GP*C)-3') Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 16 nucleotides
Theoretical weight: 5.25 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GAI 4 x GAI
Ligand SPM 4 x SPM
Ligand MG 1 x MG
1 modified residue:
Ligand 5BU 4 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 50.234Å b: 60.54Å c: 71.914Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.238
Expression system: Not provided

Quick links

Citations

6 review citations

Former orphan riboswitches reveal unexplored areas of bacterial metabolism, signaling, and gene control processes.
Sherlock et al. (2020)
5 more

1 mention without citation

Structural basis for ligand binding to the guanidine-II riboswitch.
Reiss et al. (2017)