› 5vu4

X-ray diffraction
2.25Å resolution

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225Q/L226A mutant in complex with 6'-SLN

Released:
DOI: 10.2210/pdb5vu4/pdb
PDB entry 5vu4 coloured by chain, front view.
PDB entry 5vu4 coloured by chain, side view.
PDB entry 5vu4 coloured by chain, top view.
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Primary publication:
Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin.
Wu NC, Xie J, Zheng T, Nycholat CM, Grande G, Paulson JC, Lerner RA, Wilson IA
Cell Host Microbe 21 742-753.e8 (2017)
PMID: 28618270

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187534 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (7 distinct):
Hemagglutinin HA1 chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 323 amino acids
Theoretical weight: 35.54 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 27-345; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 174 amino acids
Theoretical weight: 20.04 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 346-519; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: SIA, GAL
Carbohydrate polymer
2 bound ligands:
Ligand NAG 4 x NAG
Ligand SIA 2 x SIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 210.344Å b: 131.41Å c: 72.345Å
α: 90° β: 98.65° γ: 90°
R-values:
R R work R free
0.182 0.18 0.208
Expression system: Trichoplusia ni

Quick links

Citations

9 review citations

A Perspective on the Structural and Functional Constraints for Immune Evasion: Insights from Influenza Virus.
Wu et al. (2017)
8 more

1 mention without citation

Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin.
Wu et al. (2017)