5wqe

X-ray diffraction
3.13Å resolution

Crystal structure of Alicyclobacillus acidoterrestris C2c1 in complex with single-guide RNA at 3.1 Angstrom resolution

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-197012 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas12b Chain: A
RNA (60-MER) Chain: B
Molecule details ›
Chain: B
Length: 112 nucleotides
Theoretical weight: 36.37 KDa
Source organism: Alicyclobacillus acidoterrestris ATCC 49025
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 194.554Å b: 129.836Å c: 84.001Å
α: 90° β: 110° γ: 90°
R-values:
R R work R free
0.239 0.238 0.268
Expression systems:
  • Escherichia coli
  • Not provided