› 5x2h

X-ray diffraction
2.3Å resolution

Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACA PAM)

Released:
DOI: 10.2210/pdb5x2h/pdb
PDB entry 5x2h coloured by chain, front view.
PDB entry 5x2h coloured by chain, side view.
PDB entry 5x2h coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Primary publication:
Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems.
Yamada M, Watanabe Y, Gootenberg JS, Hirano H, Ran FA, Nakane T, Ishitani R, Zhang F, Nishimasu H, Nureki O
Mol Cell 65 1109-1121.e3 (2017)
PMID: 28306506

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-509992 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 835 amino acids
Theoretical weight: 96.8 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P897 (Residues: 1-480, 642-984; Coverage: 84%)
Gene names: Cj1523c, cas9
Sequence domains:
Target DNA strand Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.47 KDa
Non-target DNA strand Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
sgRNA Chain: B
Molecule details ›
Chain: B
Length: 93 nucleotides
Theoretical weight: 30.02 KDa

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 102.039Å b: 103.911Å c: 134.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.231
Expression system: Escherichia coli

Quick links

Citations

29 review citations

The next generation of CRISPR-Cas technologies and applications.
Pickar-Oliver et al. (2019)
28 more

5 mentions without citation

Type II-C CRISPR-Cas9 Biology, Mechanism, and Application.
Mir et al. (2018)
4 more