› 6cts

X-ray diffraction
2.5Å resolution

PROPOSED MECHANISM FOR THE CONDENSATION REACTION OF CITRATE SYNTHASE. 1.9-ANGSTROMS STRUCTURE OF THE TERNARY COMPLEX WITH OXALOACETATE AND CARBOXYMETHYL COENZYME A

Released:
DOI: 10.2210/pdb6cts/pdb
PDB entry 6cts coloured by chain, front view.
PDB entry 6cts coloured by chain, side view.
PDB entry 6cts coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Proposed mechanism for the condensation reaction of citrate synthase: 1.9-A structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme A.
Karpusas M, Branchaud B, Remington SJ
Biochemistry 29 2213-9 (1990)
PMID: 2337600

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 47.32 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P23007 (Residues: 1-433; Coverage: 100%)
Gene name: CS
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments


Cofactor: Ligand CIC 1 x CIC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 104Å b: 78.1Å c: 58.3Å
α: 90° β: 78.9° γ: 90°
R-values:
R R work R free
0.11 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

"Strong" hydrogen bonds in chemistry and biology.
Perrin et al. (1997)
3 more