› 6gsv

X-ray diffraction
1.75Å resolution

FIRST-SPHERE AND SECOND-SPHERE ELECTROSTATIC EFFECTS IN THE ACTIVE SITE OF A CLASS MU GLUTATHIONE TRANSFERASE

Released:
DOI: 10.2210/pdb6gsv/pdb
PDB entry 6gsv coloured by chain, front view.
PDB entry 6gsv coloured by chain, side view.
PDB entry 6gsv coloured by chain, top view.
Source organism: Rattus rattus
Primary publication:
First-sphere and second-sphere electrostatic effects in the active site of a class mu gluthathione transferase.
Xiao G, Liu S, Ji X, Johnson WW, Chen J, Parsons JF, Stevens WJ, Gilliland GL, Armstrong RN
Biochemistry 35 4753-65 (1996)
PMID: 8664265

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Mu 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 25.8 KDa
Source organism: Rattus rattus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04905 (Residues: 2-218; Coverage: 100%)
Gene name: Gstm1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GPS 2 x GPS
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 86.62Å b: 68.54Å c: 80.37Å
α: 90° β: 105.74° γ: 90°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily.
Sheehan et al. (2001)
7 more

5 mentions without citation

Interaction preferences across protein-protein interfaces of obligatory and non-obligatory components are different.
De et al. (2005)
4 more