› 6rhv

X-ray diffraction
2.29Å resolution

Crystal structure of mouse CD11b I-domain (CD11b-I) in complex with Staphylococcus aureus octameric bi-component leukocidin LukGH (LukH K319A mutant)

Released:
DOI: 10.2210/pdb6rhv/pdb
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PDB entry 6rhv coloured by chain, front view.
PDB entry 6rhv coloured by chain, side view.
PDB entry 6rhv coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.
Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A
OpenAccess logo Proc Natl Acad Sci U S A 117 317-327 (2020)
PMID: 31852826

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-138708 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized leukocidin-like protein 1 Chain: G
Molecule details ›
Chain: G
Length: 309 amino acids
Theoretical weight: 35.63 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2FWP0 (Residues: 30-338; Coverage: 100%)
Gene name: SAOUHSC_02241
Sequence domains: Leukocidin/Hemolysin toxin family
Uncharacterized leukocidin-like protein 2 Chain: H
Molecule details ›
Chain: H
Length: 324 amino acids
Theoretical weight: 37.62 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2FWN9 (Residues: 28-351; Coverage: 100%)
Gene name: SAOUHSC_02243
Sequence domains: Leukocidin/Hemolysin toxin family
Integrin alpha-M Chain: C

Ligands and Environments

2 bound ligands:
Ligand DMS 6 x DMS
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P4212
Unit cell:
a: 130.494Å b: 130.494Å c: 109.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

The Promiscuous Profile of Complement Receptor 3 in Ligand Binding, Immune Modulation, and Pathophysiology.
Lamers et al. (2021)
2 more

1 mention without citation

Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.
Trstenjak et al. (2020)

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