› 6xkg

X-ray diffraction
1.55Å resolution

Crystal structure of 3-O-Sulfotransferase isoform 3 in complex with 8mer oligosaccharide with 6S sulfation

Released:
DOI: 10.2210/pdb6xkg/pdb
PDB entry 6xkg coloured by chain, front view.
PDB entry 6xkg coloured by chain, side view.
PDB entry 6xkg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deciphering the substrate recognition mechanisms of the heparan sulfate 3-O-sulfotransferase-3.
Wander R, Kaminski AM, Xu Y, Pagadala V, Krahn JM, Pham TQ, Liu J, Pedersen LC
OpenAccess logo RSC Chem Biol 2 1239-1248 (2021)
PMID: 34458837

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 31.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9Y663 (Residues: 139-406; Coverage: 66%)
Gene names: 3OST3A1, HS3ST3A, HS3ST3A1, UNQ2551/PRO6180
Sequence domains: Sulfotransferase domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BDP, SGN, IDS
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BDP, SGN, IDS, NGY
Carbohydrate polymer
4 bound ligands:
Ligand NPO 2 x NPO
Ligand A3P 2 x A3P
Ligand NA 2 x NA
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 133.76Å b: 65.012Å c: 92.235Å
α: 90° β: 124.735° γ: 90°
R-values:
R R work R free
0.164 0.164 0.183
Expression system: Escherichia coli K-12

Quick links

Citations

3 citation in other articles

Increased 3-O-sulfated heparan sulfate in Alzheimer's disease brain is associated with genetic risk gene HS3ST1.
Wang et al. (2023)
2 more

4 mentions without citation

Passive Immunotherapy Against SARS-CoV-2: From Plasma-Based Therapy to Single Potent Antibodies in the Race to Stay Ahead of the Variants.
Strohl et al. (2022)
3 more