› 7mdh

X-ray diffraction
2.4Å resolution

STRUCTURAL BASIS FOR LIGHT ACITVATION OF A CHLOROPLAST ENZYME. THE STRUCTURE OF SORGHUM NADP-MALATE DEHYDROGENASE IN ITS OXIDIZED FORM

Released:
DOI: 10.2210/pdb7mdh/pdb
PDB entry 7mdh coloured by chain, front view.
PDB entry 7mdh coloured by chain, side view.
PDB entry 7mdh coloured by chain, top view.
Source organism: Sorghum bicolor
Primary publication:
Structural basis for light activation of a chloroplast enzyme: the structure of sorghum NADP-malate dehydrogenase in its oxidized form.
Johansson K, Ramaswamy S, Saarinen M, Lemaire-Chamley M, Issakidis-Bourguet E, Miginiac-Maslow M, Eklund H
Biochemistry 38 4319-26 (1999)
PMID: 10194350

Function and Biology Details

Reaction catalysed: 
(S)-malate + NADP(+) = oxaloacetate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-148109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase [NADP] 1, chloroplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 375 amino acids
Theoretical weight: 40.87 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17606 (Residues: 55-429; Coverage: 87%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 17 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2221
Unit cell:
a: 145.424Å b: 153.944Å c: 160.042Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Redox regulation: a broadening horizon.
Buchanan et al. (2005)
12 more