› 7mql

X-ray diffraction
1.6Å resolution

AAC(3)-IIIa in complex with CoA and neomycin

Released:
DOI: 10.2210/pdb7mql/pdb
PDB entry 7mql coloured by chain, front view.
PDB entry 7mql coloured by chain, side view.
PDB entry 7mql coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural elucidation of substrate-bound aminoglycoside acetyltransferase (3)-IIIa.
Zieliński M, Blanchet J, Hailemariam S, Berghuis AM
OpenAccess logo PLoS One 17 e0269684 (2022)
PMID: 35921328

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside N(3)-acetyltransferase III Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 274 amino acids
Theoretical weight: 29.95 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P29808 (Residues: 1-271; Coverage: 100%)
Gene name: aacC3
Sequence domains: Aminoglycoside 3-N-acetyltransferase

Ligands and Environments


Cofactor: Ligand COA 4 x COA
2 bound ligands:
Ligand RIO 4 x RIO
Ligand FMT 8 x FMT
1 modified residue:
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER METALJET
Spacegroup: P21212
Unit cell:
a: 130.584Å b: 90.882Å c: 100.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.199
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structural elucidation of substrate-bound aminoglycoside acetyltransferase (3)-IIIa.
Zieliński et al. (2022)