› 1efk

X-ray diffraction
2.6Å resolution

STRUCTURE OF HUMAN MALIC ENZYME IN COMPLEX WITH KETOMALONATE

Released:
DOI: 10.2210/pdb1efk/pdb
PDB entry 1efk coloured by chain, front view.
PDB entry 1efk coloured by chain, side view.
PDB entry 1efk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a closed form of human malic enzyme and implications for catalytic mechanism.
Yang Z, Floyd DL, Loeber G, Tong L
Nat Struct Biol 7 251-7 (2000)
PMID: 10700286

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent malic enzyme, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 584 amino acids
Theoretical weight: 66.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23368 (Residues: 1-584; Coverage: 100%)
Gene name: ME2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
2 bound ligands:
Ligand MG 4 x MG
Ligand MAK 4 x MAK
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 229.6Å b: 118.6Å c: 113.1Å
α: 90° β: 109.6° γ: 90°
R-values:
R R work R free
0.218 0.218 0.301
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Malo-ethanolic fermentation in Saccharomyces and Schizosaccharomyces.
Volschenk et al. (2003)
48 other articles

5 mentions without citation

Binding leverage as a molecular basis for allosteric regulation.
Mitternacht et al. (2011)
4 more