› 1f8u

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF MUTANT E202Q OF HUMAN ACETYLCHOLINESTERASE COMPLEXED WITH GREEN MAMBA VENOM PEPTIDE FASCICULIN-II

Released:
DOI: 10.2210/pdb1f8u/pdb
PDB entry 1f8u coloured by chain, front view.
PDB entry 1f8u coloured by chain, side view.
PDB entry 1f8u coloured by chain, top view.
Source organisms:
Primary publication:
Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II.
Kryger G, Harel M, Giles K, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL
Acta Crystallogr D Biol Crystallogr 56 1385-94 (2000)
PMID: 11053835

Function and Biology Details

Reaction catalysed: 
Acetylcholine + H(2)O = choline + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-142974 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Acetylcholinesterase Chain: A
Molecule details ›
Chain: A
Length: 583 amino acids
Theoretical weight: 64.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P22303 (Residues: 32-614; Coverage: 100%)
Gene name: ACHE
Sequence domains:
Structure domains: alpha/beta hydrolase
Fasciculin-2 Chain: B
Molecule details ›
Chain: B
Length: 61 amino acids
Theoretical weight: 6.77 KDa
Source organism: Dendroaspis angusticeps
UniProt:
  • Canonical: P0C1Z0 (Residues: 1-61; Coverage: 100%)
Sequence domains: Snake toxin cobra-type
Structure domains: CD59

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: R32
Unit cell:
a: 151.11Å b: 151.11Å c: 247.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.227
Expression system: Homo sapiens

Quick links

Citations

14 review citations

Acetylcholinesterase--new roles for an old actor.
Soreq et al. (2001)
13 more

11 mentions without citation

The chemistry of snake venom and its medicinal potential.
Oliveira et al. (2022)
10 more