› 1js3

X-ray diffraction
2.25Å resolution

Crystal structure of dopa decarboxylase in complex with the inhibitor carbidopa

Released:
DOI: 10.2210/pdb1js3/pdb
PDB entry 1js3 coloured by chain, front view.
PDB entry 1js3 coloured by chain, side view.
PDB entry 1js3 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural insight into Parkinson's disease treatment from drug-inhibited DOPA decarboxylase.
Burkhard P, Dominici P, Borri-Voltattorni C, Jansonius JN, Malashkevich VN
Nat Struct Biol 8 963-7 (2001)
PMID: 11685243

Function and Biology Details

Reaction catalysed: 
5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-550828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic-L-amino-acid decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 486 amino acids
Theoretical weight: 54 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P80041 (Residues: 1-486; Coverage: 100%)
Gene name: DDC
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand SO4 4 x SO4
Ligand 142 2 x 142
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE D41A
Spacegroup: P62
Unit cell:
a: 154.36Å b: 154.36Å c: 86.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.144 0.144 0.187
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Biogenic amines and polyamines: similar biochemistry for different physiological missions and biomedical applications.
Medina et al. (2003)
19 more

20 mentions without citation

Current Advances on Structure-Function Relationships of Pyridoxal 5'-Phosphate-Dependent Enzymes.
Liang et al. (2019)
19 more